Practical LC-MS/MS Method Development
Saturday, March 7, 2026 8:30 AM to 5:00 PM · 8 hr. 30 min. (America/Chicago)
Short Course Office - Room 211
Short Course
Pharmaceutical & Biologics
Information
This course offers practical training for the practicing scientists. It will take the participants step-by-step through the concepts and techniques to develop LC-MS/MS methods. The emphasis is on practical issues associated with developing LC-MS/MS methods for small molecules. It also emphasizes problem-solving skills with examples encountered in the pharmaceutical industry and other fields. This course will provide the participants with an updated overview and a solid working knowledge of LC-MS/MS. The participants will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guides, and key applications. After this course, the participants will be able to independently develop their own LC-MS/MS methods. New technologies and techniques, such as monolithic chromatography and hydrophilic interaction liquid chromatography (HILIC) will be presented. Since some of the participants are from the pharmaceutical industry, which is regulated by GLP and GMP, some regulation and validation concepts will be introduced.
Day of Week
Saturday
Session Number
SC-7273
Application
Pharmaceuticals
Methodology
Liquid Chromatography/LCMS
Primary Focus
Application
Short Course Information
Course Level
Intermediate
Duration
Full Day Course
Learning Objective
Fully understand the principles of LC-MS/MS systems
Apply the LC-MS/MS principles to develop your own LC-MS/MS methods
Course Outline
1.HPLC separation principles - advantages and limitations
Important concepts - retention factor, column efficiency, separation factor and master resolution
How pH affects the separation – application of Henderson-Hasselbach equation
How to select buffer and optimize the pH for the buffer based on pKa values
How to separate polar molecules using HILIC
How to develop and transfer HPLC methods to UHPLC/UPLC methods
2.Introduction to mass spectrometry
Why the combination of LC with MS is a perfect marriage
Important concepts – charged species, mass resolution and mass accuracy
What kind of ionization should be selected – ESI, APPI or APCI
Mass analyzers: quadrupole, time of flight, ion trap and orbitrap
How to evaluate and compensate for matrix effects on MS ionization
Advantages and limitation of mass ionizer: single and triple quadrupole, TOF, ion trap and Q-exactive
How to develop an MS or MS-MS method
How to perform a qualitative and quantitative analysis using LC-MS/MS
Target Audience
This one-day course is intended for analytical chemists, supervisors, lab managers, and researchers using LC-MS/MS. It will benefit the scientists ranging from college graduates to professionals in the analytical field, especially in the pharmaceutical industry and biotech.
Early Fee (before Jan 29, 2026)
$900.00
Full Fee (after Jan 29, 2026)
$1100.00
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Speakers
Perry Wang
FDA