Intermediate Volume Liners for Splitless Injections on Narrow-bore Columns
Sunday, March 2, 2025 11:40 AM to 12:00 PM · 20 min. (America/New_York)
Room 109A
Oral
Environment & Energy
Information
Background: Sample introduction to narrow-bore columns can often result in widened peaks when using a standard liner (ex: 4 mm ID) due to inefficient sample transfer to the column head. Smaller ID liners (ex: 2 mm ID) allow for more efficient transfer but are limited by injection volume. An intermediate volume (IV) liner was developed to increase liner volume while reducing the risk of backflash and minimizing peak widening during sample transfer. Chromatographic performance of semivolatiles, PAHs, and pesticides were evaluated using different liner dimensions and injection volumes.
Methods: The IV liner (3 mm ID) was compared to larger (4 mm ID) and smaller ID (2 mm ID) liners for three classes of analytes: (1) semivolatiles, (2) PAHs, and (3) pesticides. Compounds were separated using narrow-bore (≤0.18 mm ID) columns. Average peak area, height, width, resolution, and tailing were determined by performing six 0.5 µL injections on the smaller and IV liners, and six 1 µL injections on the larger (4 mm ID) and IV liners. Peak characteristics and resolutions were compared between liner dimensions and injection volumes within each compound class. Reproducibility was compared between injection volumes and between liner volumes, across analyte classes.
Results: Of the 50 evaluation points, 43 (86%) showed the IV liner provided improved equivalent performance compared to smaller and larger volume liners. The most notable benefits were observed for reproducibility, peak area, and height, which are critical for accurate identification and quantification. Improvements were more varied for width, resolution, and symmetry.
Conclusion: The IV liner allows additional sample to be injected onto narrow-bore columns without risking backflash or compromising peak characteristics. Comparisons between liner volumes demonstrates how the IV liner can improve critical peak characteristics, improving overall separation and quantitation for various applications.
Methods: The IV liner (3 mm ID) was compared to larger (4 mm ID) and smaller ID (2 mm ID) liners for three classes of analytes: (1) semivolatiles, (2) PAHs, and (3) pesticides. Compounds were separated using narrow-bore (≤0.18 mm ID) columns. Average peak area, height, width, resolution, and tailing were determined by performing six 0.5 µL injections on the smaller and IV liners, and six 1 µL injections on the larger (4 mm ID) and IV liners. Peak characteristics and resolutions were compared between liner dimensions and injection volumes within each compound class. Reproducibility was compared between injection volumes and between liner volumes, across analyte classes.
Results: Of the 50 evaluation points, 43 (86%) showed the IV liner provided improved equivalent performance compared to smaller and larger volume liners. The most notable benefits were observed for reproducibility, peak area, and height, which are critical for accurate identification and quantification. Improvements were more varied for width, resolution, and symmetry.
Conclusion: The IV liner allows additional sample to be injected onto narrow-bore columns without risking backflash or compromising peak characteristics. Comparisons between liner volumes demonstrates how the IV liner can improve critical peak characteristics, improving overall separation and quantitation for various applications.
Day of Week
Sunday
Session or Presentation
Presentation
Session Number
OR-07-07
Application
Method Development
Methodology
Gas Chromatography/GCMS
Primary Focus
Application
Morning or Afternoon
Morning
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