HPLC and UHPLC for Practicing Scientists: Fundamentals, Method Development, and Troubleshooting

This intermediate, all-inclusive 1-day workshop will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns, applications, and best practices in method development and troubleshooting of HPLC and UHPLC (ultra-high-pressure liquid chromatography). The focuses are on the pharmaceutical analysis of small-molecule drugs, regulated analysis, and research in bioscience for laboratory scientists.

1. Fundamentals A. Concepts Advantages and limitations, chromatographic modes, Concepts, Mobile phase factors, and Operating conditions. B. HPLC Columns and Trends Packing characteristics, bonded phases, the van Deemter equation, and Trends. C. HPLC Instrumentation The System, Pump, Autosampler, UV/Visible detector, Mass spectrometer, Data Chromatography System, and UHPLC (overview, benefits). 2. HPLC Applications, Method Development and Validation A. HPLC applications Pharmaceutical, biopharmaceuticals, food, environmental, chemical, and bioseparations B. HPLC Method Development The traditional strategy, the 3-pronged template, and the universal generic gradient approach. C. An overview of Method Validation for regulated analysis 3. HPLC Operation and Troubleshooting A. HPLC Operation Mobile phase/sample preparations and the best practice in HPLC operation B. HPLC Troubleshooting Diagnosing and solving problems (pressure, baseline, peak, data performance)

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want an updated overview of the fundamentals of (u)HPLC in pharmaceutical analysis and other applications. A basic understanding of chemistry and some hands-on experience with HPLC are assumed.