A Suitable Size Exclusion Column For Efficient Adeno-Associated Virus Aggregate Analysis

Size exclusion is a non adsorptive chromatography technique that is commonly utilized for Aggregate analysis. The primary goal of such technique is to resolve the key component from its aggregates reliably and accurately. Since this is a size based separation that relays on a compound’s hydrodynamic volume, having optimal pore size and inert chemistry are important to promote size based separation and to avoid unwanted secondary interaction. In addition, optimal mobile phase and column dimension are important for aggregate recovery and for high throughput. In this study, we have explored several parameters that are necessary for optimal aggregate analysis of various modalities to provide the best resolution and recovery of aggregates.