Single-Cell Analysis by Mass Spectrometry

Recent advances in separations and mass spectrometry technology have extended made possible the in-depth biochemical profiling of single cells. A rapidly growing number of laboratories seek to adapt single-cell mass spectrometry across a broad swath of biology. Technologies based on automated sample preparation, liquid chromatography and capillary electrophoresis as well as mass spectrometry have enabled ultra-high sensitive quantification of hundreds of metabolites and thousands of proteins in various cell types. These studies are revealing previously unknown (and unexpected) paradigms about the molecular organization of the cell. Yet there are many challenges associated with the design, execution and data analysis of single-cell proteomics. This symposium will bring together experts across academia and industry to fulfill the following aims: (1) Update the scientific community of technological advances with a focus on study design, execution, and data analysis; (2) disseminate single-cell methods and protocols to promote technological adaptation with a focus on recent community-wide standards established in single-cell mass spectrometry proteomics; (3) identify remaining challenges in single-cell proteomics; (4) recruit new members to the field.